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Henry Stewart, 1st Lord Methven (c. 1495 – 1552) was Master of the Scottish Artillery and third, and last husband, of Margaret Tudor, eldest daughter of Henry VII of England and Elizabeth of York.

Margaret Tudor (28 November 1489 – 18 October 1541) was Queen of Scotland from 1503 until 1513 by marriage to James IV. She then served as regent of Scotland during her son's minority, and fought to extend her regency. Margaret was the eldest daughter and second child of Henry VII of England and Elizabeth of York, and the elder sister of Henry VIII. By her line, the House of Stuart eventually acceded to the throne of England and Ireland, in addition to Scotland.

Margaret married James IV at the age of 13, in accordance with the Treaty of Perpetual Peace between England and Scotland. Together, they had six children, though only one of them reached adulthood. Margaret's marriage to James linked the royal houses of England and Scotland, which a century later resulted in the Union of the Crowns. Following the death of James IV at the Battle of Flodden in 1513, Margaret, as queen dowager, was appointed as regent for their son James V. A pro-French party took shape among the nobility, urging that the king's closest male relative, John Stewart, Duke of Albany, should replace Margaret as regent. In seeking allies, Margaret turned to the Douglases, and in 1514 she married Archibald Douglas, 6th Earl of Angus, with whom she had one daughter, Margaret Douglas. Margaret's marriage to Angus alienated other powerful nobles and saw Albany take her place as regent. In 1524, Margaret, with the help of the Hamiltons, removed Albany from power in a coup d'état while he was in France, and was recognised by Parliament as regent, then later as chief counsellor to James V when he came of age.

In 1527, Pope Clement VII approved Margaret's annulment from Angus. The following year, she married Henry Stewart, whom the King created Lord Methven. Through her first and second marriages, Margaret was the grandmother of both Mary, Queen of Scots, and Henry Stuart, Lord Darnley.